Jun Y Lee

6495350, Dec 17, 2002

Dec 23, 1999

09/472066

Jun E. Lee - North Potomac MD
Ayoub Rashtchian - Gaithersburg MD

Invitrogen Corporation - Carlsbad CA

C12Q 168

435 912, 435 6, 435 71, 435 911, 435 9151, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

6767724, Jul 27, 2004

Aug 21, 2002

10/224334

Jun E. Lee - North Potomac MD
Ayoub Rashtchian - Gaithersburg MD

Invitrogen Corporation - Carlsbad CA

C12P 1934

435 912, 435 6, 435 71, 435 911, 435180, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

7883871, Feb 8, 2011

Jun 7, 2004

10/861469

Jun E. Lee - North Potomac MD, US
Ayoub Rashtchian - Gaithersburg MD, US

Life Technologies Corporation - Carlsbad CA

C12P 19/34
C07H 21/04

435 9121, 435 912, 536 231, 536 232

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

20040209276, Oct 21, 2004

Sep 15, 2003

10/661819

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsville MD, US
Jun Lee - San Diego CA, US

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12N015/74

435/006000, 435/069100, 435/091200, 435/199000, 435/252300, 435/320100, 536/023200

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

20070141592, Jun 21, 2007

Jul 25, 2006

11/492194

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsille MD, US
Jun Lee - San Diego CA, US

C12Q 1/68
C07H 21/04
C12P 21/06
C12N 9/22
C12N 15/86

435006000, 435069100, 435199000, 435456000, 435325000, 536023200

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

20120021464, Jan 26, 2012

Dec 28, 2010

12/979899

Michael D. SMITH - Rockville MD, US
Robert Jason POTTER - San Marcos CA, US
Gulshan DHARIWAL - Potomac MD, US
Gary F. GERARD - Frederick MD, US
Kim ROSENTHAL - Laytonsville MD, US
Jun E. LEE - San Diego CA, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

C12P 19/34
C12N 9/12

435 9151, 435194

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

59035439, May 11, 1999

May 21, 1996

8/652020

Jun Guy Lee - Gaithersburg MD
Young I. L. Kim - Gaithersburg MD
Sung Min Song - Gaithersburg MD

Samsung Electronics Co., Ltd. - Suwon

H04J3/06

370216

In a clock system having active and standby clocks for synchronizing cell data flow in a data system, and for preventing cell data loss during active to standby clock switching, a prevent circuit maintains the active clock cycle signal during the active/standby clock switching to ensure the proper setup time before the active clock is disabled. The prior standby clock will become active during the maintenance period, thereby providing the proper setup time before and after switching, permitting system redundancy without loss of data.