Ji Whan Lee

8492101, Jul 23, 2013

Apr 16, 2010

12/761721

Ji Hoon Lee - Gaithersburg MD, US

Luminescent MD, LLC - Gaithersburg MD

G01N 33/53
G01N 33/533

435 71, 435 792

A chemiluminescent enzyme immunoassay method for quantifying antigen or antibody using 1,1′-oxalyldiimidazole (ODI) derivative or 1,1′-oxalyldisodium benzoate (ODB) derivative chemiluminescence (CL) detection was developed. Also, various enzymes were quantified using ODI derivative or DOB derivative CL detection. Fluorescent compound formed from a substrate (non-fluorescent compound) through the enzyme assay methods emitted CL when the fluorescent compound received energy from high-energy intermediate formed in ODI derivative or ODB derivative CL reaction.

20120237957, Sep 20, 2012

Mar 16, 2012

13/422650

Ji Hoon LEE - Gaithersburg MD, US

G01N 33/566
G01N 33/53

435 792, 435 71

Additives capable of controlling the reaction rate between substrate and enzyme to form a dye in enzyme assays with absorbance, chemiluminescence, and fluorescence are effective tools for optimizing enzyme assays. A dye can be formed rapidly from the reaction between enzyme and substrate in the presence of a catalyst. Using relatively high concentration of dye formed from the rapid reaction, trace levels of analytical materials can be quantified using absorbance, chemiluminecence and fluorescence detection. A dye can be formed from a relatively slow reaction between enzyme and substrate in the presence of a surfactant such as Triton X-100 and β-cyclodextrin. Using relatively low concentration of dye from the slow reaction, a high concentration of analytical material can be quantified without any dilution using absorbance, chemiluminescence, and fluorescence.

20210270833, Sep 2, 2021

Oct 15, 2018

16/755805

Ji Hoon LEE - Gaithersburg MD, US
Carol T. LEE - Gaithersburg MD, US

G01N 33/569
G01N 33/553
G01N 33/58

A biosensor for detecting an influenza A virus in a sample is disclosed, which includes: an influenza A virus antibody immobilized on a surface of Au—FeOcomposite; where the antibody binds with the influenza A virus in the sample, which converts 4-methylumbelliferyl-N-acetyl-α-D-neuraminic acid (MUNANA) to 4-methylumbelliferone (4-MU), where the 4-MU emits green light at pH of 5.5-6.5; and wherein the 4-MU emits blue light at pH of 9.3-11.3. In the biosensor, 1,1′-oxalyldiimidazole chemiluminescence (ODI-CL) reagent may be utilized to emit the blue and green lights.

20200182889, Jun 11, 2020

Jul 17, 2017

16/316346

Ji Hoon LEE - Gaithersburg MD, US

G01N 33/86
G01N 21/76
C09K 11/07

A chemiluminescent biosensor for detecting a coagulation factor in a blood sample including: a fluorogenic substrate for the coagulation factor, where the fluorogenic substrate includes a fluorescent dye; and a quencher conjugated with the fluorogenic substrate. The biosensor rapidly and accurately detects a coagulation factor in a blood sample including whole blood or plasma, thereby useful for minimizing or eliminating any reversal effect of anticoagulants.

20180275120, Sep 27, 2018

Apr 15, 2016

15/566557

Ji Hoon LEE - Gaithersburg MD, US

G01N 33/53
C12N 15/115

A method detects a target material in a sample, and includes: providing an aptasensor having a first aptamer capable of binding with hemin; a second aptamer capable of binding with the target material; and a nucleotide linker connecting the first aptamer and the second aptamer, adding hemin to the sample, contacting the aptasensor with the sample including hemin to bind the first aptamer with hemin and the second aptamer with the target material; adding a substrate for 1,1′-oxalyldiimidazole (ODI) chemiluminescence (CL), and measuring intensity of CL.

20150301036, Oct 22, 2015

Dec 24, 2014

14/582691

- Gaithersburg MD, US
Ji Hoon LEE - Gaithersburg MD, US

LUMINESCENT MD, LLC - Gaithersburg MD

G01N 33/543

Additives capable of controlling the reaction rate between substrate and enzyme to form a dye in enzyme assays with absorbance, chemiluminescence, and fluorescence are effective tools for optimizing enzyme assays. A dye can be formed rapidly from the reaction between enzyme and substrate in the presence of a catalyst. Using relatively high concentration of dye formed from the rapid reaction, trace levels of analytical materials can be quantified using absorbance, chemiluminecence and fluorescence detection. A dye can be formed from a relatively slow reaction between enzyme and substrate in the presence of a surfactant such as Triton X-100 and β-cyclodextrin. Using relatively low concentration of dye from the slow reaction, a high concentration of analytical material can be quantified without any dilution using absorbance, chemiluminescence, and fluorescence.

20150276725, Oct 1, 2015

Oct 10, 2013

14/434610

- Gaithersburg MD, US
Ji Hoon Lee - Gaithersburg MD, US

LUMINESCENT MD, LLC - Gaithersburg MD

G01N 33/53
G01N 33/543
G01N 33/58

A chemiluminescent immunoassay for sensing an analyte in a sample includes an oligonucleotide, a buffer solution, a chemiluminescent reagent, and a micro-particle or a nano-particle. An analyte in a sample is detected by conjugating an oligonucleotide with (i) a fluorescent dye or a fluorescent polystyrene bead and (ii) a micro-particle or a nano-particle; mixing the conjugated oligonucleotide and a chemiluminescent reagent; and measuring light intensity generated as a result of mixing the conjugated oligonucleotide and chemiluminescent reagent. The oligonucleotide advantageously captures and detects the analyte without the requirement of an anti-body. The micro-particle or nano-particle removes excess oligonucleotide without the requirement of washing.