US Patent:
20150159133, Jun 11, 2015
Inventors:
Fan Yang - Frederick MD, US
Huan Tran - Boyds MD, US
Thomas Fellner - Gaithersburg MD, US
Assignee:
LONZA WALKERSVILLE, INC. - Walkersville MD
International Classification:
C12N 5/0793
Abstract:
Methods are disclosed for the initiation and differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) into motor neuron progenitor cells (MNPs). Methods are also disclosed for the cryopreservation of MNPs. The methods particularly relate to the simple, efficient, scalable, and reproducible generation, and subsequent frozen maintenance, of MNPs for downstream therapeutic applications. The methods can be used for the production of MNPs from various lines of hESCs and iPSCs.