David W Zhang

6569647, May 27, 2003

Apr 23, 1999

09/299217

David Y. Zhang - Jamaica NY
Margaret Brandwein - Jamaica Estates NY
Terence C. H. Hsuih - Long Island City NY

Mount Sinai School of Medicine of New York University - New York NY

C12P 1934

435 912, 435 6, 435 911, 436 94, 536 231, 536 243, 536 2433

An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes. Alternatively, separate capture and amplification probes may be used which form continuous full-length or circular probes, and may be directly detected or amplified using a suitable amplification technique, e. g.

6593086, Jul 15, 2003

Dec 1, 2000

09/728265

David Y. Zhang - Jamaica NY

Mount Sinai School of Medicine of New York University - New York NY

C12Q 168

435 6, 435 911, 435 912, 436 94, 536 231, 536 243, 536 2433

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

RE38442, Feb 24, 2004

Mar 2, 2001

09/798641

David Y. Zhang - Jamaica NY
Margaret Brandwein - Jamaica NY

Mount Sinai School of Medicine - New York NY

C12Q 170

435 5, 435 71, 435 6, 435 79, 435 912, 536 231, 536 243, 536 2432, 536 2433

An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplifications probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes. Alternatively, separate capture and amplification probes may be used which form continuous full-length or circular probes, and may be directly detected or amplified using a suitable amplification technique, e. g.

6855523, Feb 15, 2005

Dec 4, 2002

10/309438

David Y. Zhang - Jamaica NY, US
Margaret Brandwein - Jamaica Estates NY, US
Terence C. H. Hsuih - Long Island City NY, US

Mount Sinai School of Medicine of New York University - New York NY

C12P019/34
C12Q001/68
C07H021/02
C07H021/04
C07H021/00

435 912, 435 6, 435 911, 435 9151, 536 231, 536 237, 536 2372, 536 243, 536 2433, 536 253

An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes. Alternatively, separate capture and amplification probes may be used which form continuous full-length or circular probes, and may be directly detected or amplified using a suitable amplification technique, e. g.

8632999, Jan 21, 2014

Jun 13, 2003

10/461848

David Y. Zhang - Jamaica NY, US

C12P 19/34
C12Q 1/68
C07H 21/02
C07H 21/04

435 9151, 435 61, 435 611, 435 612, 435 911, 435 912, 536 231, 536 243, 536 2433

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

7361489, Apr 22, 2008

Nov 21, 2003

10/719480

David Y. Zhang - Jamaica NY, US
Wandi Zhang - New York NY, US
Jizu Yi - Bayside NY, US

Mount Sinai School of Medicine of New York University - New York NY

C12P 19/34
C12Q 1/68
C07H 21/02
C07H 21/04
C07H 21/00

435 912, 435 6, 435 911, 536 231, 536 243, 536 2433, 536 253

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

20030175706, Sep 18, 2003

Oct 15, 2001

09/978261

David Zhang - Jamaica NY, US

C12P019/34
C12Q001/68

435/006000, 435/091200

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

20030236205, Dec 25, 2003

Jun 21, 2002

10/176515

David Zhang - Jamaica NY, US
Wandi Zhang - New York NY, US

A61K048/00
C12Q001/68
A61K039/395

514/044000, 424/178100, 514/008000, 435/455000, 435/006000

The present invention relates to a hybridization signal amplification method (HSAM) that can be used to form nanostructures for use in drug delivery and diagnostics and may comprise molecules aimed at a specific target cell of interest. The nanostuctures may be used to treat infectious diseases and physiological disorders such as proliferative, genetic, neurological or metabolic disorders. The nanostructures of the invention comprise nucleic acid molecules having affinity pairs incorporated into their structure. These affinity pairs are formed from ligand and ligand binding moieties that bind to nucleic acid molecules. This bound entity is a complex, web-like structure that serves as a matrix or framework for delivery of therapeutic or diagnostic agents. Since the nanostructures of the invention are comprised of biocompatible and biodegradable materials, such as nucleic acid molecules and proteins, they provide a safe and easily degradable delivery system.