Bo O Wang

20210406414, Dec 30, 2021

Jun 8, 2021

17/341596

- Dallas TX, US
Bo Wang - Dallas TX, US
Wenzhong Zhang - Dallas TX, US

G06F 30/10
G06F 17/16

Systems and methods for computing interactions of charge sources embedded in three dimensional (3D) layered media. At least one of the methods includes decomposing the Green's function representing the potential caused by a charge source into a free space component and a plurality of reaction components; generating, for each reaction component of the plurality of reaction components, a multipole expansion (ME) operator and a multipole to local (M2L) translation operator; performing, for each reaction component of the plurality of reaction components, a convergence analysis of an ME of that reaction component; defining, for each reaction component, polarization charge sources based on the convergence analysis and combining the polarization charges sources with the charge sources; and computing, using a fast multipole method (FMM), interactions of the charge sources based on the polarization charge sources, the ME operators, and the M2L translation operators.

20160060643, Mar 3, 2016

Sep 1, 2015

14/842671

Bo Wang - Tempe AZ, US
Weiwen Zhang - Tianjin City, CN
Deirdre Meldrum - Phoenix AZ, US

Arizona Board of Regents on behalf of Arizona State University - Scottsdale AZ

C12N 15/82

Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome of sp. PCC 6803 (hereafter 6803) and expressed in that harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in , the integrative plasmids were extracted and used for transformation of 6803. The results showed that expression of slr0214 in the integrative-plasmid-harboring cells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of 6803.