Ben D Johnson

6372106, Apr 16, 2002

Jul 26, 1999

09/361485

Ben F. Johnson - Palo Alto CA
Eric S. Nordman - Palo Alto CA

Applera Corporation - Foster City CA

G01N 2726

204455, 204451, 204601, 204605

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

7169276, Jan 30, 2007

Feb 13, 2002

10/075404

Ben F. Johnson - Palo Alto CA, US
Eric S. Nordman - Palo Alto CA, US

Applera Corporation - Foster City CA

G01N 27/447

204451, 204455

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

20060160122, Jul 20, 2006

Feb 15, 2006

11/355872

Michael Harrold - San Mateo CA, US
Aldrich Lau - Palo Alto CA, US
Ben Johnson - Palo Alto CA, US
Gilbert Amparo - Hayward CA, US
Frank Mercer - Belmont CA, US

Applera Corporation - Foster City CA

C12Q 1/68

435006000

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

20100196912, Aug 5, 2010

Apr 9, 2010

12/757888

Michael P. Harrold - San Mateo CA, US
Aldrich N.K. Lau - Palo Alto CA, US
Ben F. Johnson - Palo Alto CA, US
Gilbert P. Amparo - Hayward CA, US
Frank W. Mercer - Belmont CA, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

C12Q 1/68
C07H 1/06
C09K 3/00
G01N 27/26

435 6, 536 254, 252184, 204451

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

20120305838, Dec 6, 2012

Jun 5, 2012

13/489381

Michael P. Harrold - San Mateo CA, US
Aldrich N.K. Lau - Palo Alto CA, US
Ben F. Johnson - Palo Alto CA, US
Gilbert P. Amparo - Hayward CA, US
Frank W. Mercer - Belmont CA, US

APPLIED BIOSYSTEMS, LLC - Carlsbad CA

B01J 39/18
B01J 39/20
B01J 41/14
B01J 41/12

252184, 521 28

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

58913130, Apr 6, 1999

Nov 8, 1995

8/554247

Ben F. Johnson - Palo Alto CA
Steven M. Menchen - Fremont CA
Will Bloch - San Mateo CA

The Perkin-Elmer Corp. - Foster City CA

C25B 700

204451

A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.

54683653, Nov 21, 1995

Sep 22, 1993

8/125623

Steven M. Menchen - Fremont CA
Mitchell A. Winnik - Toronto, CA
Ben F. Johnson - Palo Alto CA

Applied Biosystems, Inc. - Foster City CA

C25B 700

204299R

A viscous electrophoresis separation medium is disclosed. The medium is formed by a matrix formed by a copolymer composed of hydrophilic polymer segments having selected, substantially uniform segment lengths, and a plurality of hydrophobic polymer segments carried on, and spaced from one another by the hydrophilic polymer segments. Also disclosed is an electrophoresis method which employs the separation medium, and novel copolymers used in forming the medium.

60516364, Apr 18, 2000

Mar 9, 1999

9/264757

Ben F. Johnson - Palo Alto CA
Steven M. Menchen - Fremont CA
Will Bloch - San Mateo CA

The Perkin-Elmer Corporation - Foster City CA

C08K 5151
C08K 53415

524 98

A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.