Amy F Liu

6365410, Apr 2, 2002

May 19, 1999

09/314847

Volker Schellenberger - Palo Alto CA
Amy D. Liu - Mountain View CA
Olga V. Selifonova - Los Altos CA

Genencor International, Inc. - Palo Alto CA

C12N 1574

435488, 435440, 4352523, 43525233, 435243, 4353201

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

6436632, Aug 20, 2002

Oct 4, 2001

09/970578

Volker Schellenberger - Palo Alto CA
Amy Deming Liu - Mountain View CA

Genencor International, Inc. - Palo Alto CA

C12Q 100

435 4, 4352831, 435 29, 435 30, 422 50, 422 681

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study.

6706503, Mar 16, 2004

Oct 23, 2001

10/037677

Volker Schellenberger - Palo Alto CA
Amy D. Liu - Mountain View CA
Olga V. Selifonova - Los Altos CA

Genencor International, Inc. - Palo Alto CA

C12P 104

435170, 4352523, 435440, 435441, 435233, 435234, 435195, 435183, 4351738, 435471, 435479, 435481, 435482, 435 691

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

7125695, Oct 24, 2006

Nov 20, 2003

10/719571

Volker Schellenberger - Palo Alto CA, US
Amy D. Liu - Mountain View CA, US
Olga V. Selifonova - Los Altos CA, US

Genencor International, Inc. - Palo Alto CA

C12P 1/04
C12P 21/06
C12N 15/00
C12N 9/90
C12N 9/00
C12N 15/74
C12N 1/20
C12N 15/01

435170, 435 691, 435440, 435441, 435233, 435234, 4352523, 435183, 4351738, 435195, 435479, 435481, 435482, 435471

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

7666360, Feb 23, 2010

Aug 20, 2002

10/223893

Volker Schellenberger - Palo Alto CA, US
Amy Deming Liu - Mountain View CA, US

BioTrove, Inc. - Woburn MA

B01L 3/00

422102, 422104

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study.

8236542, Aug 7, 2012

Jun 3, 2009

12/477887

Thijs Kaper - Palo Alto CA, US
Bradley R Kelemen - Menlo Park CA, US
Amy D. Liu - Sunnyvale CA, US

Danisco US Inc. - Palo Alto CA

C12N 9/14
C12P 19/00
C12P 7/06

435195, 435 72, 435161

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

8569034, Oct 29, 2013

Oct 28, 2008

12/740782

David A. Estell - San Francisco CA, US
Ronaldus W. J. Hommes - Haarlem, NL
Amy D. Liu - Sunnyvale CA, US
Andrew Shaw - San Francisco CA, US

Danisco US Inc. - Palo Alto CA

C12N 9/48
C11D 3/386

435212, 510306

The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.

20050059074, Mar 17, 2005

Oct 20, 2004

10/969104

Volker Schellenberger - Palo Alto CA, US
Amy Liu - Mountain View CA, US

C12Q001/68
G01N033/53
C12N015/85

435006000, 435007100, 435455000

A spatially fixed library of mutated cells and a method for creating such a library. The library is created by creating a set of cells having a genetic diversity, causing the cells to multiply and express phenotypes, preparing a solution containing the cells diluted in a medium, populating a plurality of through-holes of a testing plate with the solution such that surface tension holds the solution in respective through-holes, and analyzing the phenotypes of the cells in the solution on a hole-by-hole basis. Cells expressing specified phenotypes may then be retrieved.