Search

Patricia A Kasila

from Windham, NH
Age ~68

Patricia Kasila Phones & Addresses

  • 4 Misty Meadow Rd, Windham, NH 03087 (603) 560-9115
  • 16 Kent St, Windham, NH 03087 (603) 437-0841 (603) 437-9204
  • Estero, FL
  • Chelmsford, MA

Skills

Immunoassays • Lifesciences • Technology Transfer • Biochemistry • Biotechnology • Molecular Biology • Fda • Elisa • Gmp • Validation • Sop • Cell Biology • Immunology • Genomics • Biopharmaceuticals • Drug Discovery • Assay Development • Life Sciences

Industries

Biotechnology

Resumes

Resumes

Patricia Kasila Photo 1

Patricia Kasila

View page
Location:
21700 Southern Hills Dr, Estero, FL 33928
Industry:
Biotechnology
Skills:
Immunoassays
Lifesciences
Technology Transfer
Biochemistry
Biotechnology
Molecular Biology
Fda
Elisa
Gmp
Validation
Sop
Cell Biology
Immunology
Genomics
Biopharmaceuticals
Drug Discovery
Assay Development
Life Sciences

Publications

Us Patents

Solid Phase Cell-Based Assay

View page
US Patent:
57390010, Apr 14, 1998
Filed:
Oct 29, 1996
Appl. No.:
8/744718
Inventors:
Beverly Ann Brown - Winchester MA
Patricia Ann Kasila - Windham NH
Assignee:
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
G01N 33543
C12Q 168
US Classification:
435 793
Abstract:
A solid phase cell-based assay is described which can be used to study cellular and biochemical processes in living cells which are responding to a stimulus by evaluating cell-related analytes without the need to attach the cell to the solid phase, without the need to radioactively label the cell either by incorporating a radioactive label into the cell or introducing a radioactive label onto the cell surface and without the need to employ cell culture techniques.

Cell Enumeration Immunoassay

View page
US Patent:
56587457, Aug 19, 1997
Filed:
Feb 17, 1995
Appl. No.:
8/390598
Inventors:
Richard Alfred Greene - Westford MA
Patricia Ann Kasila - Windham NH
Mark Norman Bobrow - Lexington MA
Assignee:
E. I. Du Pont de Nemours and Company - Wilmington DE
International Classification:
C01N 33566
US Classification:
435 724
Abstract:
This invention relates to a cell enumeration immunoassay which uses a calibrated standard, i. e. , a substance which behaves like the sample under study and the concentration of which can be correlated to the concentration of the cells. This immunoassay is an efficient alternative to flow cytometry.

Assay Device And Immunoassay

View page
US Patent:
51378043, Aug 11, 1992
Filed:
May 10, 1988
Appl. No.:
7/192087
Inventors:
Richard A. Greene - Westford MA
Patricia A. Kasila - Windham NH
Assignee:
E. I. Du Pont de Nemours and Company - Wilmington DE
International Classification:
C12Q 170
G01N 3353
US Classification:
435 5
Abstract:
This invention relates to an improved assay device and assay for detecting or quantitating the presence or absence of a substance in a sample. The device has multiple layers comprising a permeable layer (a) having a capture reagent attached to less than the entire membrane, a selectively permeable layer (b) which does not allow assay reagents to pass through (b) and an absorbent layer (c). Layer (b) is in communication with layers (a) and (c). Layer (b) has at least one hole extending therethrough and the hole or holes are directly below the capture reagent in layer (a). The area of the hole or the combined area of the holes is less than the area covered by the capture reagent. This invention also relates to a method of reducing background color development in an absorbent layer of an assay device.

Enzyme Assay Using A Solid Phase Substrate

View page
US Patent:
59725958, Oct 26, 1999
Filed:
Dec 16, 1998
Appl. No.:
9/212211
Inventors:
Patricia Kasila - Windham NH
Thomas R. Mullinax - Newton MA
Michael E. Bembenek - Burlington MA
Mark N. Bobrow - Lexington MA
Assignee:
NEN Life Science Products, Inc. - Boston MA
International Classification:
C12Q 100
C12Q 144
C12Q 134
C12Q 148
US Classification:
435 4
Abstract:
This invention relates to a hydrophobic layer formed on a solid support, the hydrophobic layer incorporating by hydrophobic interactions an amphipathic enzyme substrate labeled with a reporter on its hydrophilic region, and the use of the same to conduct various enzyme assays without extraction steps.

Assay Device And Immunoassay

View page
US Patent:
53914783, Feb 21, 1995
Filed:
Feb 21, 1992
Appl. No.:
7/838662
Inventors:
Richard A. Greene - Westford MA
Patricia A. Kasila - Windham NH
Assignee:
E. I. Du Pont de Nemours and Company - Wilmington DE
International Classification:
C12Q 170
G01N 2100
US Classification:
435 5
Abstract:
This invention relates to an improved assay device and assay for detecting or quantitating the presence or absence of a substance in a sample. The device has multiple layers comprising a permeable layer (a) having a capture reagent attached to less than the entire membrane, a selectively permeable layer (b) which does not allow assay reagents to pass through (b) and an absorbent layer (c). Layer (b) is in communication with layers (a) and (c). Layer (b) has at least one hole extending therethrough and the hole or holes are directly below the capture reagent in layer (a). The area of the hole or the combined area of the holes is less than the area covered by the capture reagent. This invention also relates to a method of reducing background color development in an absorbent layer of an assay device.
Patricia A Kasila from Windham, NH, age ~68 Get Report