Karen E Broglie

6376749, Apr 23, 2002

Feb 25, 1999

09/257894

Karen E. Broglie - Landenberg PA
Natalie L. Hubbard - Wilmington DE
Theodore M. Klein - Wilmington DE
Jonathan E. Lightner - Airville PA

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 1582

800284, 800278, 800286, 800287, 8003201, 435101, 435468

The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.

6392120, May 21, 2002

Jun 30, 1999

09/345214

Karen E. Broglie - Landenberg PA
Jonathan Edward Lightner - Airville PA

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 1582

800284, 800278, 800285, 800286, 800287, 800320, 8003201, 8003203, 435 691, 435101, 435468

The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.

6429358, Aug 6, 2002

Nov 5, 1999

09/403618

Karen E. Broglie - Landenberg PA

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 1582

800284, 800278, 536 236, 435 691, 435101, 435210, 4353201, 435419, 435468

This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn pullulanase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn pullulanase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of corn pullulanase in a transformed host cell.

6570008, May 27, 2003

Jan 15, 2001

09/743980

Karen E. Broglie - Landenberg PA
Jonathan Edward Lightner - Airville PA

E. I. du Pont de Nemours and Company - Wilmington DE

C08B 3300

536102, 800284, 800278, 800285, 800286, 800287, 800320, 8003201, 8003203, 435 691, 435101, 435468

The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endospern tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.

6620987, Sep 16, 2003

Nov 15, 2000

09/713273

Stephen M. Allen - Wilmington DE
Karen E. Broglie - Landenberg PA
Karlene H. Butler - Newark DE
Robert F. Cressman - Wilmington DE

E. I. DuPont de Nemours Company - Wilmington DE

A01H 500

800298, 4353201, 435 711, 435419, 800284, 536 232, 536 236

An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having starch R1 phosphorylation activity, or the complement of the nucleotide sequence. Plant transformation and regeneration methods using the polynucleotides. Vectors and recombinant DNA constructs containing the polynucleotides. Cells, plants, and seeds containing the recombinant DNA constructs. Methods for isolating polypeptides having starch R1 phosphorylation activity.

6930226, Aug 16, 2005

May 2, 2002

10/138075

Karen E. Broglie - Landenberg PA, US
Karlene H. Butler - Newark DE, US
Leslie T. Harvell - Newark DE, US
Jonathan E. Lightner - Mulino OR, US

E. I. du Pont de Nemours and Company - Wilmington DE

C12N015/82
C12N015/29
A01H005/00
A01H005/10
C12P019/04

800284, 800278, 800286, 435 691, 435101, 4353201, 435419, 435468, 536 232, 536 236, 536 245

This invention relates to isolated nucleic acid fragments encoding granule-bound starch synthase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the granule-bound starch synthase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the granule-bound starch synthase in a transformed host cell.

7244839, Jul 17, 2007

Mar 26, 2003

10/397954

Richard W. Pearlstein - Newark DE, US
Karen E. Broglie - Landenberg PA, US
Christopher F. Hines - Middletown DE, US

E. I. du Pont de Nemours and Company - Wilmington DE

C08B 31/00
C08B 33/00
C08B 35/00
C07H 19/00
C07H 21/00

536102, 536105, 536107, 536108, 536111, 536115, 536120, 536124

The present invention is directed to a starch comprising a novel percentage of actual amylose. A grain comprising the starch is also embodied by the present invention. The present invention is also directed to a plant comprising the novel starch.

7619133, Nov 17, 2009

Jul 20, 2007

11/780499

Karen E. Broglie - Landenberg PA, US
Karlene H. Butler - Newark DE, US
Alexandre da Silva Conceição - Wilmington DE, US
Travis James Frey - Huxley IA, US
James A. Hawk - Newark DE, US
Dilbag Singh Multani - Urbandale IA, US
Petra Johanna Christina Cecilia Wolters - Wilmington DE, US

Pioneer Hi-Bred International, Inc. - Johnston IA
E.I. du Pont de Nemours and Company - Wilmington DE
University of Delaware - Newark DE

C12N 15/82
C12N 15/87
A01H 5/00

800279, 800301, 800278

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen , which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen , and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to and resistant to stalk rot.