Inventors:
Lansing J. Stewart - Bainbridge Island WA, US
Alex Burgin - Kingston WA, US
John W. Walchli - Seattle WA, US
Kathryn Hjerrild - Bainbridge Island WA, US
Assignee:
deCODE biostructures, Inc. - Bainbridge Island WA
International Classification:
G01N 33/50
Abstract:
Pieces of software and databases are used to facilitate the design and synthesis of genes. The synthesis techniques allow identification, quantification, transcription, translation, and manipulation of portions of the gene sequence represented as 0's and 1's in a computing system. These pieces of software and databases compile constraint information provided by a user to create synthetic genes to express proteins lacking disordered or variable regions and containing surface mutations that promote solubility and/or crystallization. Regions of the gene sequence that do not form stable structures can be removed and replaced by a short biological linker to improve crystallization of proteins.